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The method that is certainly routinely employed for the differential fractionation of liver tissue serves to be a practical illustration of this technique and is particularly shown diagrammatically in Figure 12-4.

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Pre-heat oven to 350°File. After oven is preheated to 350°File; area foil pan with foil lid on baking sheet. Spot baking sheet with foil pan in oven on Middle rack.

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The instantaneous sedimenta­tion amount of a particle throughout centrifugation is prevent­mined by 3 forces:

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The microsomal supernatant is often called the soluble period on the cells, or cytosol, and involves soluble proteins, soluble nucleic acids, soluble polysac­charides, lipid droplets, together with other minute particles. Inside the process just described the liver tissue is sepa­rated into 4 key fractions.